Animal Data Shows Fisetin to be a Surprisingly Effective Senolytic

It is exciting to see animal data arrive for some of the potentially senolytic compounds that may turn out to destroy enough senescent cells in mammals to be worth using as first generation rejuvenation therapies. As a reminder, the accumulation of senescent cells is one of the causes of aging; countless cells become senescent every day in our bodies, but near all are destroyed. A tiny fraction linger to cause significant harm through the inflammatory signal molecules that they secrete. If these errant cells can be removed, then inflammatory diseases and numerous aspects of aging can be turned back to some degree. The results in mice stand head and shoulders above all of the other approaches to aging in terms of reliability and breadth of benefits.

Some senolytic compounds have been tested in animals, but a larger body of candidate senolytic drugs are presently only accompanied by cell study data. The ability to selectively destroy senescent cells in a petri dish does little more than indicate potential; there is a significant rate of failure in medical research and development for compounds with promising cell data, and any number of reasons as to why they may not work well enough in tissues or otherwise turn out to be infeasible for use in animals and humans. Fisetin was one such senolytic candidate with cell study data only, and I had not viewed it as a likely prospect. It is a flavonoid, and the one other well-known possibly senolytic flavonoid turned out not to be useful on its own - though it is helpful as a part of a combination treatment.

Given that, results from the recent animal study of fisetin noted here greatly exceed expectations, surprisingly so. Fisetin appears about as effective in mice as any of the current top senolytics, such as the chemotherapeutics dasatinib and navitoclax. Per the data in the open access paper below, dosing with fisetin destroys 25-50% of senescent cells depending on organ and method of measurement. The dose level is large in absolute terms, as one might expect for a flavonoid. For aged mice and a one-time treatment, the researchers used 100 mg/kg daily for five days. The usual approach to scale up estimated doses from mouse studies to initial human trials leads to 500 mg per day for five days for a 60kg human.

Given the wealth of new results emerging these days, it seems to me that people focused on self-experimentation, open human trials, and investigative mouse studies in this field should be moving to focus on combination therapies. Consider a combination of fisetin, dasatinib, quercetin, piperlongumine, and FOXO4-DRI - multiple different mechanisms to provoke apoptosis that are all hitting senescent cells at the same time. The goal would be to see if it is possible to engineer a significantly higher level of clearance of senescent cells than any of these senolytics can achieve on their own. This seems like a plausible goal, and may turn out to present meaningful competition to efforts such as those of Oisin Biotechnologies and other groups developing more sophisticated senolytic therapies that should have high rates of clearance.

Researchers Have Discovered How to Slow Aging

As people age, they accumulate damaged cells. When the cells get to a certain level of damage they go through an aging process of their own, called cellular senescence. The cells also release inflammatory factors that tell the immune system to clear those damaged cells. A younger person's immune system is healthy and is able to clear the damaged cells. But as people age, they aren't cleared as effectively. Thus they begin to accumulate, cause low-level inflammation and release enzymes that can degrade the tissue.

Researchers found a natural product, called fisetin, reduces the level of these damaged cells in the body. They found this by treating mice towards the end of life with this compound and see improvement in health and lifespan. "These results suggest that we can extend the period of health, termed healthspan, even towards the end of life. But there are still many questions to address, including the right dosage, for example." One question they can now answer, however, is why haven't they done this before? There were always key limitations when it came to figuring out how a drug will act on different tissues, different cells in an aging body. Researchers didn't have a way to identify if a treatment was actually attacking the particular cells that are senescent, until now.

Fisetin is a senotherapeutic that extends health and lifespan

A panel of flavonoid polyphenols was screened for senolytic activity using senescent murine and human fibroblasts, driven by oxidative and genotoxic stress, respectively. The top senotherapeutic flavonoid was tested in mice modeling a progeroid syndrome carrying a p16INK4a-luciferase reporter and aged wild-type mice to determine the effects of fisetin on senescence markers, age-related histopathology, disease markers, health span and lifespan. Human adipose tissue explants were used to determine if results translated.

Of the 10 flavonoids tested, fisetin was the most potent senolytic. Acute or intermittent treatment of progeroid and old mice with fisetin reduced senescence markers in multiple tissues, consistent with a hit-and-run senolytic mechanism. Fisetin reduced senescence in a subset of cells in murine and human adipose tissue, demonstrating cell-type specificity. Administration of fisetin to wild-type mice late in life restored tissue homeostasis, reduced age-related pathology, and extended median and maximum lifespan.


Powerful senolytic with apparently no adverse side effects, easily attainable and inexpensive, what are we waiting for? It's amazing something so simple could have such a huge impact on world life expectancy.

Posted by: Corbin at October 3rd, 2018 6:07 PM

Check for the Mayo human trials of fisetin. Dosage is 20 mg/kg/day for 2 consecutive days. I've already done my trial.

Posted by: Proud Daddy at October 3rd, 2018 6:14 PM

@Proud Daddy
What brand of Fisetin supplement did you use?

Posted by: Stephan at October 3rd, 2018 6:31 PM

From what I hear most of the human senolytic trials are using higher doses that you'd get by the usual method of scaling up from the relevant mouse studies. I haven't had a chance to chat to someone who knows why this is the case.

Posted by: Reason at October 3rd, 2018 6:38 PM

I am hoping to be using Senolytics within 2 years. Would anyone hazard a guess when data would show the safety and effectiveness of Fisetin? This sounds like an effective 1st gen (and low cost) Senoytic treatment. I am hoping we are talking months and not years for some definitely data results.

BTW, I looked on Amazon for what is available and cost for Fisetin.

Posted by: Robert at October 3rd, 2018 8:35 PM

The safety is kinda already proven. It is a widely available supplement and it is considered to be a neuroprotector without proven ill side effects, if taken in moderation. Probably you will need to take a cocktail of different weak senolitics including dasatinib and navitoclax to get a detectable effect. I am not sure whether fasting for 2 weeks, can give a better effect than the weak senolitics, but that's the poors man autophagy booster that is available even now...

I waiting for OISINs mice study to finish. p53+p16 targets seem to be doing much better than the control group. My hopes are high on their approach. And i am rather skeptical about finding a strong senolytic within the existing supplements. If it was available, somebody already should have seen the remarkable effects by now.

Posted by: cuberat at October 3rd, 2018 10:43 PM

Hi there, hoping this pans out into something tangible (in humans). I think we should curb our enthusiasm (not to be pessimistic) but because mouse study results don't always translate 1:1 to human (more often than not, they don't translate at all because we have different 'specie master plans/blueprints'), with that said a 20% extension in mice might give us a 5-10 year boost in health and lifespan. I'm saying this because we already get some (though not much in general) senolytics in the food we eat or beverages drunk (red wines/red grapes, certain fruits and vegetables). This means that it may be end up being redundant (or it may synergize but I doubt it because of pathway redundancy (i.e the therapies are just emulating what these senolytics are already doing), long lived people drank (and thus consumed senolytic mimics) red wine, like centenarians (yet other centenarians did not drink once ounce of wine and ate plenty of crapfood (that kills everyone else) and Still lived to centenarian. Demonstrating, clearly, that its 'some placebo' effect there and 'mild' improvement in health - but it's genetics (epigenetics specifically, which act in concert with/to damages but superced damage as a signature you cannot just erase by removing damage/even reprogramming proved it you can't erase cell age memory) that determines if you reach a 100 or not (you inherited family genetic is also behind this, it touches the intrinsic genomic epidevelopment; which is different than the extrinsic one molded by external factors). Taking senolytics will improve external epiaging, which relates to health status (senolytics reduce SASP/p16p53/IL6/TNF, basically reduce inflammation and general stress, oxidative stress too. But, this is still something different than your DNA intrinsic epigenetic aging clock which relies on cell passaging (something uncoupled from replicative senescence/Hayflick limit and telomeres attrition; they are just independent measures of your health but the one that dictates chronological lifespan is in the methylome landscape; damages just accelerate that (as seen in Hutchison Gilford Progeria) but 'regular' 'healthy' aging is dependent on cell passaging (can be uncoupled from senescence); unlike accelerated aging (HGPS/Werner) which relies on replicative senescence acceleration). In my mind, the cell 'age memory' is what determines longevity, this signature is (so far) irreversible/permanent once written (the cell Never forgets its age and thus 'act' appropriately to its age despite being reverted to immature state ('Age 0')).

I hope they make these therapies available soon, we have been talkinb about SENS since....god knows whwen, sh*T needs to happen, we are aging and people don't give 2fcks about these therapise, they prefer botox and beautiful cremes (or even, goodness sake, surgery (f...!) of their face), face lift, and look 20 again in the mirror; who doesn't?

There is a saying (I.Newton i think) :

''Work expands as it completes itself and 'fill its time' to be made/accomplished''

which basically, means, it could take long(er), still. And we ain't gettin younger. I know we still have time and have to 'tough' it but the more time passes the more I realize we were too eager/too optimisic/too innocent, there are so many obstacles, people that don't want longevity extension, peoplpe who love to 'die' (aka fatalists) and force us 'to accept our fate and be good person with a conscience not selfish', people screaming 'overpopulation' and bs, FDA/health regulation hurdles and sh...I fear we will wait AT least another 15 years before we see ANYthing remotely 'good'. The 'news' you hear are falling on deaf tone ears and they baby steps - that scientists have been making for what? like 40 years now, baby baby babysteps one after another...- you're dead (too late), missed the boat.
It is excrutiatingly slow AF. We don't have much time (those say, hey we have 30-40 yaers ahead of us, keep on saying that, and times goes, 'you said that 10 years before??'...OK, I'm exaggerating and going on a ranty tangent a bit (sorry)). I just feel that the biogerontology domain is both its own accomplishment and its bane. Its accomplishing Great Things, yet at the same time, not much either 'in real time'; in very long time yes, not in short 'real time'. I know that thatis the 'speed' we can get and it takes huge efforts hence why so slowmo, but it's also normal for people to get fed up and just say fck it...and leave it all altogether. It,s like this Grand Project, that may never happen, when we are older, and*t..(then) what? (we're fckd/we thought all along that this grand project would save us). That is why I try to curb enthusiasm and be more real in terms of advancement with this (I am now thinking 20 years sounds about right before anything super duper big happens/ultrastagnation the name of the game (small development don't count much if they are not marketed to public and Happen tangible/be sold to humans after trials), 20 years, in 20 years I will remember I spoke about that 20 years BEFORE...and still to same point roughly).

Just a 2 cents.

Posted by: CANanonymity at October 4th, 2018 12:03 AM

Regarding the idea that the beneficial effects of fisetin should have already been seen, I have been wondering about this myself.

If we assume that the effect size in humans is a ~10% gain in longevity, and then only after years of regular use starting at least in mid life - this effect might not be evident from uncontrolled anecdotes alone. And it would take a long, decently powered study to observe this in the clinic - one that as far as I can tell hasn't ever been attempted. There are a mere 5 results on for "fisetin," 3 of which were just started at Mayo and all of which were posted after 2016.

Now if we were talking about curcumin (183 results spanning many years) then I might be more skeptical...

Posted by: Will at October 4th, 2018 1:21 AM

@CANanonymity - a lot more impassioned than usual, I gather you feel time's running out. I'm 55 - are you older than that? I agree that humans aren't two-legged mice - we need to find much better testing methods. Now go and put some of those 2c coins in the Swear Jar :) :)

Posted by: John C. at October 4th, 2018 2:19 AM

This is a pretty cool, slam-dunk of a paper and a great 'low-hanging' fruit given fisetin's low cost and availability.

Assuming bioavailability issues can be overcome and this is translatable, fisetin alone seems to be able to reduce senescent cell load in many tissues by ~25%. It will still rise with age, even with treatment, but the burden will be less.

No doubt combinatorial therapies will be even better.

Posted by: Mark at October 4th, 2018 6:13 AM

Fisetin might be a winner.

Fisetin is found in strawberries in higher levels than other foods and a clinical trial with 60g/day freeze-dried strawberry powder reversed pre-cancerous lesions in humans.

Posted by: Lee at October 4th, 2018 6:18 AM

Re dosage and brand. I'm using Doctor's Best fisetin via Amazon. Dosage for me is 1/2 BOTTLE each day for 2 days using the Mayo clinical trial dosage. I doubt that anyone has been taking fisetin in this manner, so it isn't surprising that nobody has experienced profound benefits from regular use.

Posted by: Proud Daddy at October 4th, 2018 7:01 AM

A significant reduction in the senecent cells count should bring immediate and quite measurable health benefits. If fisetin was that good you would be witnessing major health improvements in the users. We can have several explanations what it is not the case

1. Fisetin doesn't work for humans very well 2. The bio availability is low 3. It comes with toxic sure effects. The first option basically gives no hope with this compound. The second one, requires research to improve the bioavailability (there's room for patenting medications that improve the bioavailability and that enhance the effects). It would take years for human results. The third option in a way, suggests immediate benefits. The toxicity world imply that the effects are there but not very targeted. With proper protocol the poison can become a cure. Again, they're options to patent compounds that protect non- senecent cells

And there is one more explanation that nobody noticed so far. I am not holding my breath on this. I would be glad to be proven wrong, though

Alas, what is needed is not aligned with way
What is happening. We might be on the teaching of rechuing LEV in 10-15 years. Or cold be 50 years away. And acceleration to LEV Aaron be quite uneven. People with more mean it knowledge could be 10 years ahead of the general public. For example, we (at FA and related forums) know that dasatinib should work, and the moment we have a credibile confirmation for humans, we will find a way to obtain the compound, nevermind that it costs 200$ a a gram and requires prescription. Of course, we don't know the good protocol and it is very hard to Measure the count if cenecsent cells.

Posted by: Cuberat at October 4th, 2018 8:41 AM

@Reason: from what I can tell, Human Equivalent Dose is both inexact and controversial. It's main purpose is to start human trials without killing anybody. Effective doses are often much higher than the 1/12th of mice mg/kg HED would be.

HED is based on relative surface area. The relative rate of metabolism of a drug would seem to involve a lot of other factors.

Posted by: Proud Daddy at October 4th, 2018 8:45 AM


Do we have a theory of the damage level caused by the cenecsent cells concentration? Does it go up linearly, exponentially, less stat linearly, or does it have a more cold l complex dependency?

Is a 25% refuction enough to increase the health span? I would guess that the improvement would be more for the general population and spectacular for some isolated cases

Posted by: Cuberat at October 4th, 2018 8:48 AM

what is the right dosage for humans?

Posted by: scott emptage at October 4th, 2018 9:43 AM

@proud daddy are there any adverse effects from hugh dosages of fisetin?

Posted by: scott emptage at October 4th, 2018 10:08 AM

When purchasing some long pepper peppercorns on Amazon (after giving up on finding a good source of the root), the section titled 'Frequently Bought Together' showed a fisetin supplement; apparently people have been trying the combination.

The shipment finally arrived yesterday; I literally can't believe how good it smells. I'm hesitant to ingest it since it smells like they dunked the peppercorns in patchouli oil.

Strawberries deserve their reputation as a functional food; strawberry consumption is associated with reduced all cause mortality:

I'd be happy to contribute to a crowdfunded well-designed human trial, if such a thing is possible.

Posted by: CD at October 4th, 2018 10:26 AM

@Scott. Adverse effects of high dosages are possible but unlikely. I doubt that the Mayo Clinic doctors would use them in a trial without a high degree of certainty that they're safe. I've used the half bottle dosage for two days twice now, and noticed no I'll effects.

Also, remember that maximum safe usage doses are based on continuous use, so no current guidelines would apply.

Posted by: Proud Daddy at October 4th, 2018 10:35 AM

This story is eerily reminiscent of the Phenoxodiol saga, from the Australian company Novogen, back in the late 1990s-early 2000s

Structured off another of the 6,000+ flavanoid polyphenols, it looked great in animals and cancer models, but failed to do anything meaningful in the clinic

Turned out to be a very weak chemo-sensitizer, as most of these substances end up being in humans

Probably best to save your money and eat a lot of dietary sources of these things, or which their are 000s

Posted by: DrugDev U.S. at October 4th, 2018 10:56 AM

@proud daddy when you say 2 days do you mean 2 days every week or something?

Posted by: scott emptage at October 4th, 2018 11:03 AM

@Scott. Please go to and get the details. So far, I've repeated the 2-day regimen once - 4 months later.

Posted by: Proud Daddy at October 4th, 2018 12:28 PM

I want to thank Proud Daddy for the info, and CD for the link. Looks promising and looking forward to the clinical study results. Also, it would be nice to hear from you Proud Daddy over the course of next couple years as well for your results as well.

Posted by: Robert at October 4th, 2018 2:26 PM

@CD. I'll be getting A1C and hsCRP in November. I'll try to remember to post the results here, but I'm 77 so...

Posted by: Proud Daddy at October 4th, 2018 3:00 PM

Over and over again, treatments that work well in mice have shown to be useless in people. We, as a species, are probably close to bumping up against intrinsic age barriers, unless we develop mutations such as the antitumor mutations found in mole rats, or the multiple copies of the p53 gene found in elephants.

We had been breeding ourselves, slowly, for longevity, by having older males have children with young females. That process has pretty much stopped, but clearly we did have a drive for longevity, whereas many other species, like mice, do not have any similar longevity driven (living longer would not benefit them reproductively), which makes increasing their life-spans "low-hanging fruit."

I take care of the very elderly, and looking at their skin, muscles, etc., probably the only thing holding them together at all are those senescent cells (and the foamy cholesterol in their arteries).

I suspect that, as mammals passed through the evolutionary bottleneck at the end of the Mesozoic, we lost crucial cellular DNA repair gene complexes. We need to examine other life forms, probably aquatic ones, to find the gene complex that repairs genes better than the existing, somewhat simple-minded ones, we have now, and find some way to integrate it into our genome.

Posted by: Benjamin Wade at October 4th, 2018 3:21 PM

@Benjamin Wade: What if we with biotech could end menopause and older women got children with younger men. Would that be the same effect?

Posted by: Norse at October 4th, 2018 3:38 PM

@Benjamin Wade: Im not sure that the effect has stopped. Today I saw a women 30 with a man 40 buying an apartment. He were so much older because that were in the age segment she could find anyone with a sufficient income. Its easier today to get jobs for women (nurses), then for men, which jobs get automated. I think the effect can speed up. There should be research on it.

Posted by: Norse at October 4th, 2018 3:42 PM

@benjamin wade "what works in mice are always useless in people?

not true, there have been things that have had just as good effect in humans like in mice. i agree with the caveat, but you can never tell for sure until a propper human trial has been conducted.

lets try and be open minded before jumping to conclusions

Posted by: scott emptage at October 4th, 2018 4:04 PM

the funny thing is that the stronger the breeding effect is the slower the progress would be since postponing childbirth till older and older age will make the generations longer and longer. And dilate the periods. So for that effects to have a strong difference we need to wait a few generations. Say the first child is at the age of 35. 5 generations are already 150 years.... And probably there the effect would be to shift to having the first child at 40....

Posted by: cuberat at October 4th, 2018 4:09 PM

Looking at the clinical trial that was linked by CD, I see that it's phase 2, but I can't find the phase 1 trial. Has anyone seen it or know the results?

Posted by: Adam Hruby at October 4th, 2018 5:51 PM

@Benjamin Wade & Norse : Interesting idea about the age differences in relationships being a factor in life expectancy. Now that men are considered creepers if they date a women more than 5 years younger than themselves in the West, I wonder what impact this will have on life expectancy in a few generations time?

I'm speaking as more or less as a layman, but to the objections that this wont work on humans, in particular because we already likely get enough of these natural senolytics in our diets from various foods already, the following is from the Newsweek article on the study :

"The team also tested fisetin on human fat tissue in the laboratory, to see how the drug would interact with human cells, and not just mice cells. Since they were able to reduce senescent cells in the human fat tissue, the scientists think it's likely fisetin will work in humans. However, the amount of fisetin in fruits and vegetables isn't enough to have these benefits-scientists still need to work out the best dosage."

Posted by: The Transhumanist Runner at October 5th, 2018 12:45 AM

In a few generations I should hope we are no longer relying on the genetic crap shoot for reproduction. Longevity can be engineered in. Deciding to create a new life has always seemed to me to be more than a bit hubristic. Petulant teens do have a point - they never asked to be born. Even if a parent can guarantee a good genome, they can't guarantee that their child's life will be mostly full of joy versus suffering, if the good will outweigh the bad. In a post-singularity future, if someone wants a new person in the world, it will be possible for them to redesign themselves and they will be the only one to have to live with the outcome of their decisions. If they want another being to adore them unconditionally they can get a (possibly augmented) dog.

Posted by: CD at October 5th, 2018 10:45 AM

@Transhumanist Runner

Unfortunately, it's never just about dose, but complex pharmacokinetic and pharmacodynamic differences between species

As I said above, study the history of Phenoxodiol (one of thousands of similar compounds that have been in large scale clinical trials) to see the inherent issues of this approach

Perhaps it may have benefit as a "seno-sensitizer"

Posted by: DrugDev U.S. at October 5th, 2018 2:25 PM

I just took 3,000 mg of Fisetin, along with 1,250 mg of Quercetin. In a few days, if I've suffered no ill effects, I plan to take 1,200 mg a day for five days. If I live, I shall write a follow up comment. I know this is foolish, dangerous and urge others to use caution.

Posted by: scottalias at October 6th, 2018 1:20 PM

The Mayo study cites 20mg/kg/day for their study. This translates into 1400 mg per day for a 70 Kg human. Is the study using Fisetin in pure form, or the version you can buy off of Amazon. How may days does one it for? A week? A month?

Someone here is using "Doctor's Best". Any others?

Any more thoughts on this?

I was planning to wait a couple of years before diving into the senolytics. But I'm now thinking I might want to give this a try.

The problem I have with the D & Q combo is that the Dasatinib is not specific enough and will kill functional cells that you don't want to kill and the Quercetin is essentially useless on its own.

Posted by: Abelard Lindsey at October 6th, 2018 7:12 PM

While your are taking much higher dose than what is usually recommended , it should not be life threatening, unless you have some serious kidney/liver/metabolical problems. Now there might be some harmful side effects but I am not sure that taking it orally ensures such a high bio availability. So, of you don't do the overdose now than a couple of weeks and don't have some serious problems already, you shuold
be ok... And have some more expensive pee ;)
Of course, your milage will vary...

@Abelard Lindsey
That's the catch 22. You want to kill the senecent cells, therefore you need to use something toxic. The less toxic the compound is the fewer cells it will kill. So a generally available medicine cannot be a strong senolitic without being a portion.
If the compound is highly targeted, then you limit the effect to the senecent cells. We are not there yet. Even Oisin platform is not strong enough. At least it seems to be very targeted...

So there will be some collateral damage. And here we are threading uncharted waters. We care about the senecent cells with harmful secretion. Penally killing 1 good cell for every cenecsent one is a good tradeoff. ( I don't know about the brain, though)

As for should you try it ... It is a cost versus benefit decision. If you don't have a measurable inflammation that can be blamed on cenecsent cells then I don't see much benefit. You might have some inflammation without notifying it, though.

Dasatinib has well documented side effects and the risks should be more or less clear. We don't know so we'll what will be the side effects of the d+q combo. I would err on assuming that adding quercetin is as bad as increasing the dasatinib dose. So if you are young and healthy you could easily tolerate d+q but then there will be no benefit. While I want to see human results of this combo I cannot advise you on trying it...

Posted by: Cuberat at October 6th, 2018 9:36 PM

Abelard Lindsey
I'm using the Doctors Best brand, from Amazon.

Cuberat, I am in good health, I appreciate the concern. The supplement is quite affordable. The high dose was an impulse, but I see Proud Daddy has survived essentially the same dose split over two days.

It's been nearly twelve hours and I feel fine, quite well, in fact. No negative side effects, so far, and I feel like something is happening. Don't try this at home.

Posted by: scottalias at October 7th, 2018 12:07 AM

@Benjamin Wade

" ... clearly we did have a drive for longevity, whereas many other species, like mice, do not have any similar longevity driven (living longer would not benefit them reproductively), which makes increasing their life-spans 'low-hanging fruit.'"

It would seem to me that some of it has to do with kin selection dynamics in our ancestors -- i.e., grandpas and grandmas made significant contributions to the reproductive success of their close relatives (children and grandchildren) and thus increased the frequency of their own longevity-promoting genes in the population. Not as strong an effect as we would all like (too much death by predation, accident and infection), certainly. Mice don't help their adult offspring, at all.

Posted by: cacarr at October 7th, 2018 3:26 PM

I redid the calculation. The appropriate amount of fisetin for a 70Kg person is around 700mg/day, not the 1400mg/day I cited earlier. I found the paper this blogger linked to in a previous posting about animal to human dosage calculation and have downloaded it. I am using it to calculate appropriate dosages for various senolytics as well as other stuff that may or may not remove lipofuscin. Needless to say, the next 8-12 months will be interesting for me.

Posted by: Abelard Lindsey at October 9th, 2018 8:50 PM

I took 1400mg of the Amazon "Doctor's Best" Fisetin last night, about 2 hours before bedtime. I weigh approximately 70 Kg and my age is early 60's. Administration (rather than choking down 14 pills) was to open each pill into a glass holding approximately 8 oz, then add cold water. Very little taste, not unpleasant, a little chalky.

I plan to take an additional 1400 mg tonight about the same time, then nothing more until about 3-4 months from now.

I did notice about an hour after taking it that I had a slight burning or itching on my forearms which passed quickly.

I slept well, as usual. When I first woke up this morning, I was feeling a bit hot, like I had a mild fever. That also passed quickly.

On the positive side, for quite some weeks, I've had constant inflammation/stuffiness in my nose, particularly on the right side, which is now much improved.

I do seem to have more energy this morning, but it's too soon to tell if that's real or just my imagination.

Posted by: Pete Koziar at October 10th, 2018 9:46 AM

Day 2, second 1400mg of Amazon "Doctor's Best" taken last night, about 4 hours before bedtime.

It was taken a couple of hours earlier than the night before, so I got to see more of the immediate effects. No burning or itching on my arms, just a little on my face. I did get the hot and feverish feeling again, however. I checked my temperature by mouth, and it was only up a couple of tenths of a degree. I did, however, have some shaking/trembling of my hands.

I woke up this morning after a good night's sleep, still feeling a little hot/feverish. I also had a headache, but no more shakes.

One strange effect - I could taste the fisetin again very clearly about a half hour after getting up.

By now, two hours later, it has all passed, and I'm feeling good, with a high energy level.

I plan to wait 3 months or so before taking any more of it.

Posted by: Pete Koziar at October 11th, 2018 7:06 AM

It's been six days since I took 3,000 mg of Fisetin and I've noticed a reduction the symptoms of my BPH (Benign prostatic hyperplasia). I Googled BPH and senescent cells and found a wealth of information suggesting cellular senescence is implicated as possible contributing factor to BPH, and considerable research into polyphenols in general and Fisetin in particular for treatment of BPH.

Posted by: scottalias at October 12th, 2018 1:36 PM

It's been about a week since I took 2800 mg of Fisetin across two days.

Sinus inflammation is still much reduced from what it was prior to the dosage.

Mental acuity is hard to judge, since it's affected by so many different factors (tiredness, stress, amount of sleep, etc. etc.) but I do think I'm sharper - less episodes of searching for a word, for example.

Posted by: Pete Koziar at October 17th, 2018 11:25 AM

FA says ". Consider a combination of fisetin, dasatinib, quercetin, piperlongumine, and FOXO4-DRI - multiple different mechanisms to provoke apoptosis that are all hitting senescent cells at the same time" The study being conducted on humans will use 20mg/kg of Fisetin for two days.

Opinions needed! for a two-day zombie cell clearing:
Should fisetin be consumed on an empty stomach or with food.
Take all 1200 mg at once or in divided doses each day?
Should capsules be taken whole or dumped out and mixed with some type of oil
Should piperlongumine be taken at same time or later in day?
How much piperlongumine to go with 1200 mg of Fisetin?
How much Quercetin to take at same time or later as well?
Stop all other supplements those two days?
Should we try to get FOXO4-DRI as well (hard to do)?

Posted by: august at October 22nd, 2018 2:30 PM

I hope this thread is kept up and the likes of Pete and Scottalias continue to give weekly updates. Maybe Reason should highlight it in the sidebar or something?

I notice that fisetin quickly sold out on Amazon UK. There doesn't seem to be any way of getting hold of it now in Britain other than ordering from the USA.

I would love to perform my own self-trial. As an aging runner suffering from manifold inflammation problems that hold me back, I could provide some 'objective' anecdotal feedback if my times were to suddenly improve.

Posted by: The Transhumanist Runner at October 22nd, 2018 3:44 PM

Took 700mg Fisetin for 2 consecutive days about 1 wk ago. The very first day I noted a marked diminution of back pain which has contunued to present. Even if this is placebo effect I'll take it! The question is whether the Mayo study is a two shot deal or is this repeated monthly? Any advice pros and cons to a monthly repeat?

Posted by: Wolfman at October 25th, 2018 7:08 PM

My one concern is if large dose fisetin "purges" senescent cells, isn't it in effect similar to chemotherapy. Could fisetin kill other cell lines such as stem cells? People who undergo chemotherapy and survive their cancer in the long term can have significant side effects.

Any thoughts on why fisetin would cause apoptosis on senescent cells but not stem cells.

Some articles:
Role of Flavonoids in Future Anticancer Therapy by Eliminating the Cancer Stem Cells

Posted by: fyego at October 25th, 2018 11:00 PM

Still going strong, sinuses still seem much clearer than they were before fisetin. I do think there has also been an increase in mental acuity, less episodes of "fishing for a word," and the episodes that do occur are shorter in duration.

I'm planning on another course of treatment towards the beginning of the year. I'm shooting for one every quarter (3 months) if I can still find the stuff!

I don't know if my "1400 mg per day, two consecutive days" is any better or worst than 500 per day for 5 days - the total dosage works out to be about the same, and I tolerated the 1400 mg with no problems.

As I understand it, fisetin targets only senescent cells, not stem cells, but then again, that's the risk with self-experimentation, yes?

It does seem to me, however, that senolytics are causing senescent cells to die, which means that they have to be replaced by the division of other cells, which will bring those other cells "close to the brink." It makes me wonder, then, if there isn't a point when senolytics would push organs over that brink in a chain reaction.

Posted by: Pete Koziar at October 26th, 2018 11:22 AM

I find this paper to be somewhat disconcerting when it comes to using fisetin as a senolytic.

"The dietary flavonoids myricetin and fisetin act as dual inhibitors of DNA topoisomerases I and II in cells"

I imagine the structure of DNA topoisomerases I and II are highly conserved between mice and humans being as they're integral to DNA replication, and no side-effects from fisetin (like leukemia or something similar) were seen in the mice to my knowledge, so perhaps fisetin doesn't have such a strong effect in the body. Still, it makes me question the safety of taking fisetin in the large doses that seem necessary for it to act as a senolytic. Maybe the good outweighs the bad though?

Posted by: Adam Hruby at October 30th, 2018 3:26 PM

Tranhumanist Runner: I had some trouble getting the Fisetin delivered, but finally got it. I have decided on a course of 1,000 mg (1 gram) a day for 15 days, today will be the seventh day. I am taking it on an empty stomach. The reduced symptoms of BPH have continued, hard to say what other effects I'm having. I have no way of measuring the senescent cells in my body, so any anecdote I might relate can hardly be taken as scientific evidence. Recurring pain in my back has dissipated, but that could have any number of causes, including psychosomatic causes. I've had slight trouble getting to sleep while taking the supplement, that happened when I took the large dose and stopped the next night. I have been sleeping, just have trouble getting to sleep, I have a feeling akin to excitement that I have associated with the supplement. Mainly, I've not noticed much of a change and don't expect to for a while, months or years will tell if I have less dementia, arthritis, frailty and other age related symptoms than I would have had if I had not experimented with Fisetin.
Adam Hruby I can find no evidence to suggest that links Fisetin with leukemia, quite to the contrary, Fisetin is thought to have a chemopreventive/chemotherapeutic potential against various types of cancer. Self experimentation is, however, fraught with risks and inadvisable. You should surely wait for FDA approval, which should only take a few decades.
Pete, I am thinking an organism needs a sufficient dose for a sufficient number of days. Five days for a mouse that lives a couple of years and five days for a human that can expect to live eighty years are vastly different time periods.

Posted by: scottalias at November 1st, 2018 9:33 AM

I have been looking into the dosing in the study which works out to about 20mg per KG equivalent in humans. The one issue I see is that mouse lifespans are so much shorter. In this study, they found that one human year is equivalent to nine mice days. Sonic nice had this effect in 2 days of dosing, would that indicate that for the same effect in humans we would need to take that dose daily for just over a month? That could get rather costly. What are everyone's thoughts?

Posted by: Michelle at November 4th, 2018 1:38 PM

I purchased 4 bottles (Rejuvenation Therapeutics) on Amazon after reading the article in Science Daily, thinking to add the 100mg to my daily routine. Now I've read the (above) forum I see that many are mega-dosing for just two days or a week.

Curious how long Fisetin stays in the system, would it build up over time? Can you see any benefit to taking 100mg daily open-ended? Or must one go for the 1400mg for two days?

Posted by: Rice at November 4th, 2018 3:12 PM

I took 100mg today, broke open the capsule and let 1/4 the yellow powder dissolve on my tongue (just to make sure no allergens). No taste. 30 min later took the rest of the powder. Had a headache for an hour or so after. I rarely if ever have headaches (once a year?). Maybe just the delivery method (dissolve). Will swallow the pill tomorrow.

Posted by: Rice at November 5th, 2018 6:27 AM

We're just guessing on the dosage, and self experimentation is hardly scientific. I've taken a thousand milligrams a day for 12 days, with 750 mg of quercetin a day and plan to continue for 3 more days.

My observations so far: I have less frequent urges to urinate, a symptom of BPH, I have slept through the night couple of times, for the first time in decades, and now get up once or twice a night to pee, a marked improvement which may have something to do with Fisetin.

For a couple of years, I have had an urge to drink a lot of water, over 2.5 liters a day, which my doctor attributed to my pre-diabetic condition. I don't feel the need to drink so much water any more and will be interested in my A1C and fasting blood sugar tests in January of next year. I feel like I may no longer be pre-diabetic. I have been carefully watching my diet and exercising for a few years, so this may or may not have anything to do with fisetin. I've also added a tablespoon of Moringa a day to my diet, which might be a factor.

I'm having some trouble sleeping. It takes a while to get to sleep and I wake up early and this is something I associate with the supplement so I look forward to the end of this experiment. My appetite has increased and this is not good because I am overweight, but I feel like exercising more, and am exercising more. The trouble sleeping is not that bad and if I still feel like this had benefits I'm going to do this again next year. I like to think this experiment may have delayed the onset of arthritis, dementia and a host of other maladies and look forward to scientific testing on humans, which should begin soon.

Transhumanist Runner, I'll try to remember to update this after January 8, when I get the results from my physical, my blood work and all.

Rice, I read on another article that positive results were seen with both daily dosing and intermittent high dosing, so, who knows? I also read curcumin had a similar effect in the same initial test but was not tested in high doses on mice because fisetin killed more senescent cells in the cell cultures, but not that much more. I think I might try curcumin next month, probably with the recommended dosage.

Posted by: scottalias at November 6th, 2018 4:11 PM

My father took a 1,500 mg dose of Swanson brand fisetin on Saturday and another 1,500 mg on Sunday. He is 72 years old, weight about 170 pounds. The only reported effect on Saturday was a bit of light headedness within 30 minutes of taking the dose that lasted for around 2 hours. Sunday morning he reported fatigue that lasted all day and a bigger appetite. He did not experience the light headedness after taking the 2nd dose. Monday he reported feeling even more tired than on Saturday with the same level of appetite. The only other report of interest is that he feels he can breath better, he has asthma. I'll make follow up posts in the days ahead.

Posted by: Corbin at November 6th, 2018 4:57 PM

Guys, any success stories?

Posted by: Cuberat at November 9th, 2018 5:53 PM

@Corbin, I believe the reason why your father felt tired may be because of the mechanism of senolytics. They kill off senescent cells, which then must be purged from the body via the immune system. Because of that, they could present like a mild illness (i.e. tiredness, even more appetite to replace energy reserves) until the cells are purged.

When I took my dose, I did feel a little bit "feverish" the next morning. I'm not as old as your father, so I don't have as many senescent cells to purge, so my effects were probably milder.

Just my thinking. I'm not a biologist, but an engineer, so don't take it to the bank.

By the way, still feeling fine, nose still clearer (inflammation much reduced since before the dose), and still feel cognitively smarter.

I plan on taking the next dose in late December or early January. I'm toying with the idea of combining it with quercetin, but don't know if that's a good idea or not.

Posted by: Pete Koziar at November 9th, 2018 9:57 PM

Fisetin is good for health?

Posted by: peter at November 16th, 2018 9:38 AM

Thanks Pete for the input, that was in line with my thinking too, I took it as a good sign that the fisetin from that company was the actual product. His fatigue lasted on and off until about 4 days after he took his last dose and pretty much returned to normal. There doesn't seem to be any noticeable change in his health or appearance two weeks later, except that he maintains that his asthma has improved and that I noticed he doesn't seem to cough as much. I'll give another update if anything changes.

Posted by: Corbin at November 19th, 2018 3:00 PM

Very interesting discussions so far!
I'm also planning to do my own personal trial with a 2x 1,300mg dose.
I am still relatively young (38) and in peak physical condition (exercise most days, compete in long distance races, great CV health, no health issues to speak of etc). The prospect of turning old frightens me however, and I want to delay ageing as long as possible so that my body is still "young" by the time truly effective anti-ageing therapies come around.
Is this a foolish idea at my age, or merely likely not to make much of a difference?

Posted by: Piotr at November 20th, 2018 10:06 PM

I've been on 100mg a day for about 3 weeks. Feeling no different. Maybe you only feel something at higher megadoses. Have added it to my stack and hopefully there will be some long term gain?

Posted by: Rice at November 21st, 2018 3:17 PM

It seems to be impossible to buy Fisetin in Canada. Does anyone know a source?

Posted by: Stephan at November 22nd, 2018 9:22 AM

@Rice Thanks for the link. Unfortunately, they don't ship this item to Canada.

Posted by: Stephan at November 24th, 2018 6:06 PM

@Stephen - if you really want it you can sign up for a re-mailer service. Like or Have Amazon ship to the address and then have them ship it to you. More expensive - but if you can't get it where you are...

Posted by: Rice at November 25th, 2018 4:34 AM

@Rice Cool, thanks again!

Posted by: Stephan at November 25th, 2018 6:59 PM

Piotr-I don't think that's foolish at all, but this self experimentation with high doses of fisetin might turn out to be ill advised. It is generally recognized as safe, but at a fraction of the dosages we're using. I urge caution even if I don't practice it. I don't feel like I've suffered any ill effects. Recurring back pain has subsided, urges to urinate at night associated with BPH seem to be in remission, and I have less thirst that my doctor associated with a pre-diabetic condition. My sleep has returned to normal since I stopped the fisetin. I generally feel better and feel like exercising more. Whether this has anything to do with fisetin, I couldn't say. I plan to repeat this experiment about six months after the first try, maybe 1,500 mg a day for ten days. I will update this after January 8, 2019, when I have my labs done, and maybe around March if I do repeat the experiment.

Posted by: scottalias at November 29th, 2018 7:41 PM

I'd rather go with the mouse trial that has had positive results than with the human trial that hasn't yet been conducted! That reasoning led me to take 600 mg of fisetin for each of five days (with no noticeable effects). I'd like to caution our younger readers that the risk versus reward ratio for this protocol may not be favorable for people who haven't at least reached middle-age, at which time they may need "help" in clearing their increasing number of senescent cells.

Posted by: BRIAN VALERIE at December 10th, 2018 9:46 AM

Most fisetin supplements are derived from Rhus Succedanea, a toxic plant. Presumably, manufacturers know how to remove the toxic substances, but perhaps there could be enough left to cause symptoms at the high doses being tried by some reporting here...? The plant has some similarities to poison ivy, and one of the symptoms associated with it is a rash. So the skin irritation reported by some taking high doses *might* be due to such residual toxins, rather than to the fisetin as such.

Posted by: Lou T. at December 10th, 2018 1:09 PM

I think it is important to read the original paper, which is linked to the synopsis that many of us read on In the paper it clearly states that the results seen in mice were strongly correlated to dosage. One graph they presented showed negligible results at low dosages like the 100mg/day (ignoring body weight entirely) specified on over the counter fisetin supplements. Results improved dramatically at 10-15 mg/kg of body weight. You can also google Mayo Clinical trials to learn more of their current trial to treat frailty in elderly women over 70 and a second one that they currently recruiting patients for. Both seem to be using 20mg/kg of body weight for a couple of days at 30 day intervals for 3 months over a couple of years. I am in my second month using the same protocol as described in the Mayo Clinic trials with no negative side effects observed. Some decrease in facial wrinkles and a decrease in spider veins near my ankles has taken place. (Age 64)

Posted by: Papu at December 11th, 2018 7:35 AM

Thanks for the feedback, all.
I have not yet taken the plunge; still debating whether to proceed.
Fully appreciate the point about the risk/benefit ratio being significantly higher for someone in their late 30s than in their 60s or 70s. My thinking is that, rather than wait for senescent cells to accumulate in large numbers and then get rid off them, I would like to stop aging in its tracks (relatively speaking, anyway) and prevent large numbers of senescent cells from accumulating through sporadic administration of fisetin megadoses.
All of this is, of course, highly theoretical at this stage.

Posted by: Piotr at December 11th, 2018 8:36 PM

Pray, let us keep this very valuable thread alive with current user fisetin experience reportage.

Many thanks and praise to Reason for providing this wonderful site and his informative views.

Posted by: deusexmachina at December 16th, 2018 1:21 PM

In the two and a half months since the last dose I took, nothing bad has happened, although the constant inflammation in my nose started to come baqck about two months afterwards.

I did another dosage of 1500 mg two nights ago, and 1700 mg last night (I still had 2 x 100 mg pills left over from October). In addition, I also took "Activated Quercetin complex" each night, containing 1000 mg Quercetin Dihydrate, 645 mg of Vitamin C (not my choice, but it was in the pills), and 500 mg of Bromelain (don't know why they put that in there. Whatever). As before, I opened all the pills and emptied them into a cup and added approximately 12 oz of water. To make it more palatable, I also added half a packet of Crystal Light Pure Raspberry Lemonade (mostly sugar).

Results so far - nasal inflammation has, once again, cleared. Felt a little tired yesterday, and significantly hungrier than usual (also reported by Corbin's father). I also felt a little light-headed the first night.

Today, not tired, still hungry, and have had some interesting sharp pains in various parts of my body. They pop up rather suddenly, last for about 10-15 minutes, then go away. They're rather sharp, and not associated with any activity that would have resulted in sore muscles.

I'll pass on anything notable. In any case, I plan to take another dose in 3-4 months. I'm curious if the nasal inflammation comes back again in a couple of months.

Posted by: Pete Koziar at December 28th, 2018 12:08 PM

@Pete koziar - thanks for the update. I am thinking of taking 100mg to 200mg of Fisetin per day, not for the senolytic effects, but for the anti allergy effects. Steve Hill's article over at Leaf yesterday had some links to in vitro and in vivo experiments showing that Fisetin inhibited mast cell activation and T cell mediated late phase allergic inflammation (

Immunosuppressive effects of fisetin against dinitrofluorobenzene-induced atopic dermatitis-like symptoms in NC/Nga mice. (2014)

The hydroxyflavone, fisetin, suppresses mast cell activation induced by interaction with activated T cell membranes. (2009)

So it is not surprising that Fisetin seemed to have had an impact on your nasal inflammation. I hope will have some impact on my severe chronic allergic rhinitis (CAR).

Posted by: Jim at December 29th, 2018 2:33 AM

I took 24g (24000mg) fisetin over a period of 8 weeks, 3 g / week, no adverse effects, all blood laboratory values remained perfect and normal.

3g a day also without any adverse effect.

I am a physician, neurologist, I would say that 6 g / day (6000mg / day) would be a safe dose.

(dr. kirklands trial: 1.5 g / day).


Posted by: hans at December 30th, 2018 3:02 AM

New year came.

Ihave a question. Isaw this comment,

>Fully appreciate the point about the risk/benefit ratio being significantly higher for someone in their late 30s than in their 60s or 70s.

Mouse live more longer by taking fisetin from young age?

Posted by: hand at January 1st, 2019 9:01 PM

Thanks very much for good imformation! I want to know how to check the effect of taking fisetin, how to know the increasing of new cell! I want imformation!

Posted by: iwasaki at January 3rd, 2019 2:49 AM

This research about fisetin says,

Our study provides proof-of-concept evidence that senescent cells can cause physical dysfunction and decreased survival even in young mice, while senolytics can enhance remaining health- and lifespan in old mice.

So, there is possibly of benefits by taking fisetin from young age.

But it needs long time to prove.

Posted by: iwasaki at January 5th, 2019 6:29 PM

↑sorry. above comment is not about fisetin but another senolytic drug research!

But there is possibly that this above comment is important.

It needs long time to prove.

Posted by: iwasaki at January 5th, 2019 6:47 PM

I been following this post since it started in October. I've done a fair amount of investigation over the last few months on Fisetin. I have concluded that the data supports Fisetin as a senolytic; it's less clear if fisetin can perform its senolytic function when taken orally. The community understand that senescence cells are detrimental as the data makes it quite clear…with little room for argument.

I've started a regiment of 1.4 grams of Fisetin for two days. I've had no apparent side effects from that dosage, meaning no headache, digestive issues, lightheadedness, etc. I feel same as before I took the two doses.

The 1.4 grams matches my body weight appropriately scaled from the studies. I plan to perform monthly ingestions at 1.4 grams (two doses one day apart) for three months. At this time there is no reason to believe I will suffer any unwanted side effects. However if I do I will reassess and let the community know and I'll try to be as specific as possible. I'm a male, 55 year-old aerospace engineer in fairly good shape, not overweight, I don't work out, but I do eat fairly well…just you average "Joe". I also take NR (500 mg), MSM (4 g), Pterosilbene (300 mg),CoQ10 (200 mg) and PQQ (20 mg) every day. I have been taking these for almost a year. I don't believe my current regiment will affect/skew the results.

Currently I have no known aliments (I plan to stay that away as long as I can) so I'm not attempting to treat any ailment specifically. However, I do have slight joint discomfort upon waking and if I sit more than an hour. If you're around my age you know what I mean. I'm curious if the "achenes" reduce in intensity or go away altogether. I have no way to measure other than qualitatively. I will report on this and try to be as objective as possible knowing it could be a placebo effect.

Finally I've taken pictures of my face and spider veins. If I note any changes I will let the community know.

Posted by: Thomas at January 6th, 2019 8:41 AM

It's been a couple of months since I took 18 grams of Fisetin, spread out over more than a two week period, mostly in 1 gram (ten capsules of Doctor's Best Fisetin, six bottles) doses. As far as my doctor can tell, I have suffered no ill effects. I am no longer thirsty all the time, my fasting blood sugar is 86, my A1C is 5.4. My doctor attributes this to diet and exercise, and I have been exercising more with more intensity. I have felt like exercising more since the course of Fisetin, that could be psychosomatic or just a coincidence. I have less recurring back pain. The real surprise was my testosterone level. I've been using testosterone for nearly ten years and all of a sudden, though I was on the same dose of generic Androgel as I have been using for years, my testosterone level shot up to 1300. A month after that test, after cutting the dose in half, my level is 1049, which is a bit higher than recommended, and higher than it was at the regular dose, but not dangerously high. I have no idea if this is related to the fisetin, I've found no literature suggesting senescent cells can lower testosterone levels. All I can say about the Fisetin is I feel better, I've felt like exercising more, it may have something to do with my healthy blood sugar regulation and testosterone levels, but I take a lot of other supplements, including Nicotinamide Riboside and Pterostilbene (Elysium Basis for 2.5 years), Resveratrol (more than 3 years), Quercetin (6 months), Curcumin (just started my second month) and Berberine HCL (over a year). I'm going to do this again late spring of 2019, a similar dose of around 6 bottles over 10-15 days.

Posted by: scottalias at January 11th, 2019 11:52 AM

I fasted for 3 days during which I took each day:
900 mg fisetin powder taken from capsules dissolved in 1 tsp olive oil ( delicious when fasting)
2 caps of Life Extension senolytics formula ( high potency querciten with a dasatinib mimic)
1/2 tsp. Piperlognunmine powder dissolved in olive oil.
I am now on day 3 post fast and have felt or seen nothing unusual other than a continuous warmer than usual feeling facial skin, with no temp. increase. Breathing seems improved, like I'm taking in more oxygen. I did not have breathing problems.
I do want to mention that my eyes seemed slightly yellow after fasting day 3 so I've been taking Milk Thistle and that has resolved. LEF specifically stated on the package, do not take more than 2 caps per week, so I may have overdone it. I have no idea whether to expect to see or feel any results from this treatment but plan to repeat quarterly until we learn more.

Posted by: August at January 12th, 2019 7:18 PM

Eye opening discussion. I've been taking Quercetin 250 mg. and Fisetin 100 mg. daily. Looks like I should be taking higher doses (e.g. ≈750 mg. each for a limited number of days, ≈5 days and then a longer period of none, ≈ 1 to 3 months off to increase efficacy, if I understand correctly what most discussants are saying.

Posted by: J Bentham at January 13th, 2019 9:59 AM

After 14 years of IBD I discovered I had Celiac disease. But 1 1/2 years of gluten free diet had no effect. One dose of d+q and the IBD was immediately gone and has been now for 5 months. My theory is that the Celiac disease built up large quantities of senescent cells in the gut which continued to cause enough inflammation to continue the IBS even on a gluten free diet. For me the d+q was a life changer.

Posted by: barsell at January 14th, 2019 5:03 PM

I have Osteoarthritis around my knees. I started taking D+Q three months ago (70mg of D and 1,000mg of Q for three consecutive days / month). For the first two months, I felt fever around the knees a few days after the dosage. Actually, the knee temperature got 1.5 degrees celsius higher than that of thigh. I guess my immune system attacked the senescent cells.

However, my knees are much improved now. I feel no pain anymore when I go down the stairs. I am very impressed by the result.

Posted by: Amadeus Note at January 15th, 2019 8:27 AM

At 66, I have suffered from severe degenerative arthritis for over 30 years, with both hips replaced, and a knee. Arthritis is in my neck, shoulders and back, although I have adapted to much of the pain, so I don't let it keep me from doing what I want (within limits). I've been on Tramadol for 15 years, which takes the edge off. I have been health-conscious since my early 40s, starting different regimens of supplements, the longest of which has been Krill Oil, CoQ10 and Gingko Biloba. My blood work is excellent and I have no heart-related illness; in fact, I have always monitored blood work closely, since my dad passed at 56, due to heart disease, having had his 1st heart attack at age 36!

Currently, I am on a daily intake of NADH+, Glucosamine/Chondroitin, CoQ10, Krill Oil, CBD oil, Nicotinamide Riboside, Pterostilbene and Nattokinase. I had a noticeable increase in mental clarity with the addition of NADH+ (after 3 days), and I've been taking 100mg/day since mid-December. I am looking to add the Fisetin and deciding on the dosage. I am leaning to a dosage of 20mg/kg, which would be, based on my 50kg weight, 1 gram per day, for 2 days, repeat in 1 month. Any thoughts?

Posted by: RonP at January 22nd, 2019 1:37 PM

Thanks for sharing your experience with fisetin. Would it be possible for you to share your before fisetin lab results for blood sugar, A1C and testosterone? Also, do you have before and after lab results for creatinine, HDL & LDL cholesterol and triglycerides that you can share?

Posted by: Rick Davis at January 24th, 2019 11:29 AM

I've had genital herpes for 11 years. I had breakouts pretty consistently whenever there was any damage to my shaft (masturbation, rough sex, friction from gym pants etc). I have been on the exact same vitamin regiment for the last 22 months, same diet, exercise level etc. On average I would get 7 breakouts per year (thanks spreadsheet). Since taking Fiestin I've had exactly 0 breakouts in almost 4 months. This includes during 1 cold, 1 food poisoning, and two episodes of slight damage to my penis (sickness always brings an episode on). I have absolutely 0% proof that it's the Fisetin - just throwing this out there.

Posted by: Chris at January 26th, 2019 2:52 AM

I tried Docters best Fisetin at 12mg/kg (600mg/50kg) in 5 days. As a result, I realized clear improvement of cognitive ability after the 2nd or 3rd day. I do not believe that it is a placebo as I am taking supplements to improve cognitive abilities. Clearly it was a clearer effect than other supplements.

On the other hand, I did not feel the effect of removal of senescent cells in particular. The reason may be that I am still 39 years old, or it may be due to dose/usage.

For the latter, I am planning 20mg/kg for 6 days or 10mg/kg twice a day for 6 days.

The problem is whether Fisetin's senescent cell depletion is concentration dependent or time dependent. Does anyone know the literature suggesting that?

Posted by: masashi at January 26th, 2019 10:33 AM

This research about not fisetin but another senolytic drug research says,

Our study provides proof-of-concept evidence that senescent cells can cause physical dysfunction and decreased survival even in young mice, while senolytics can enhance remaining health- and lifespan in old mice.

So, there is possibility of benefits by taking fisetin from young age.

But no one did experiment about mouse,

and it needs long time to prove for human being.

Posted by: iwasaki at February 2nd, 2019 1:52 PM

This is my update from taking two doses of Fisetin. Please see my post above dated January 6th. Physically I see no effect. As some reported, I did have a significant improvement in allergy relief (sneezing, sinus drainage, etc.). Immediately after taking the dosages I had mild, flu-like symptoms, including a slight upset stomach and some lightheadedness, which passed after an hour or two. I also noted an increase in appetite during the two days of treatment. This too was reported by others. Other than that I have suffered no other detectable ill affect. Currently, I plan another, similar dosing in six months. One final note, the first pass I took capsules. The second time I open all the capsules in a small dish and mixed it with MCT oil (C-8 and C-10). Since Fisetin is fat soluble this worked very well. I hope this helps those who are considering self-experimentation. GLTA.

Posted by: Thomas at February 8th, 2019 8:01 AM

I take fisetin 500mg today.

I felt no sideeffect now.

If I have change about me, I will write this board.

Posted by: iwasaki at February 9th, 2019 3:58 AM

I am a 58 year old male. I have hypertension, mild arthritis and asthma.
I take supplements including CoQ10, DHEA, L-Carnosine, L-Carnitine, Alpha lipoic acid, pycnogenol, Resveratrol, and Elysium Basis.

Last weekend I took 1500 mg of Fiseten + 1000 mg of Quercetin for two consecutive days 1 hour before bedtime on an empty stomach. I skipped all of my supplements both days (but did take my blood pressure medication). I had no noticeable reaction to the megadose of Fisetin. After 1 week I believe that my asthma has moderately improved, but other than that I feel no discernible difference. I intend to repeat this dosing regime the first week in March.

Posted by: DJ at February 10th, 2019 3:08 PM

I am 48 year old.
February 9th, I took 500mg fisetin
February 10th, I took 500mg fisetin
February 11th, I took 1000mg fisetin
I felt no sideeffect now.
Fisetin is good taste.

Posted by: iwasaki at February 11th, 2019 4:52 AM

Hi All...latecomer to this conversation, but here because I have a dog with cancer that I am going to give fisetin to. Trying to figure out how much when. But in all the discussion about inflammation, I see no mention of Omega 3's. They are the top anti-inflammatory in my book (I am 74, take a lot of krill oil and test CRP often so I know they work with CRP scores <.2). Anyhow, it just seems so obvious to me and I hear nothing from others re Omega 3's. Not that krill is a sustainable resource, either....

Since the dog is different yet again from mice and humans, I do not know how to assess success. Doing many other things for him as well and he is going strong more than a year from diagnosis, but the tumors are not gone, either. Just began him on rapamycin and starting Low dose naltrexone and tagamet shortly. I'm healthy and trying to get him there again and of course anti=aging is high on my list of interests. Will report as I know anything.

Posted by: Ellie at February 14th, 2019 5:26 PM

I am 48 year old.(I am humanbeing.)
February 9th, I took 500mg fisetin
February 10th, I took 500mg fisetin
February 11th, I took 1000mg fisetin
February 12th, I took 500mg fisetin
February 13th, I took 1000mg fisetin
February 14th, I took 1000mg fisetin
I felt no sideeffect now. I have no next plan. If there is some way to measure the effect of fisetin, it is good thing. But I don't know about that. So, if someone know the method about that and write the comment, it is good thing.

Posted by: iwasaki at February 15th, 2019 4:49 AM

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